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Image Search Results
Journal: EBioMedicine
Article Title: Erythropoietin-producing hepatocellular A7 triggering ovulation indicates a potential beneficial role for polycystic ovary syndrome
doi: 10.1016/j.ebiom.2018.09.046
Figure Lengend Snippet: Improvement of the 16-h function of EPAH7 in PCOS rats. (A) Estrous cycles were detected during the eight last days after the establishment of the PCOS rat model. The panel (top-to-bottom) shows the CONTROL, CONTROL+EPHA7, DHEA, and DHEA+EPHA7 groups. D, diestrus; P, proestrus; E, estrus; M, metestrus. (B) Body weight (left) and ovarian weight (right) changes in rats. (C) Glucose tolerance test and AUC values were obtained after each daily injection (s.c.) with DHEA for 20 consecutive days. (D) Rats' serum EPHA7 levels detected by ELISA. (E) Rats' serum LH (left) and FSH (middle) levels, and LH/FSH ratio (right) detected by ELISA. (F) Rats' histological microscopic ovarian structures. The panel (left-to-right) shows the CONTROL, CONTROL+EPHA7, DHEA, and DHEA+EPHA7 groups. (G) mRNA and protein abundances of StAR and CYP11A1 in rats' ovaries detected by western blot and qRT-PCR analyses. The panel (left-to -right) shows representative images of western blot assays (the immunoreactive bands were densitometrically quantified), and relative mRNA abundances. (H) Rats' serum progesterone levels detected by ELISA. (I) mRNA and protein abundances of EPHA7, C/EBPβ, KLF4 and ADAMTS1 in the rats' ovaries. β-actin or GAPDH were used as loading controls. N = 6 per group. Blots are representative, and data are presented as means ± SEM. * P < .05, ** P < .01, *** P < .001 against the group marked in the figure or against control rats; ## P < .01 against DHEA rats.
Article Snippet: The concentrations of LH, FSH, progesterone and EPHA7 in rat sera were detected using ELISA Kits for Rat Luteinizing Hormone (LH) (Mybiosource, San Diego, USA), Rat Follicle-stimulating
Techniques: Control, Injection, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR